Study of lipid-lipid and lipid-protein interactions in relation to the organization, structure and function of biological membranes


Team of work

Maté, Sabina María
Principal Investigator
mate.sabina@gmail.com
Vázquez, Romina Florencia
Investigator
rominavazquez80@hotmail.com
Longarzo, Lucrecia 
Research Fellow
lucrecialongarzo@hotmail.com
   


Collaborators from other institutions

International


Goñi, Félix M.
Unidad de Biofísica-Centro Mixto, Consejo Superior de Investigaciones Científicas, Universidad del País Vasco/Euskal Herriko Unibertsitatea, Bilbao, España.

Muñoz-Garay, Carlos
Instituto de Cs. Físicas (ICF), UNAM, México

Oliveira, Osvaldo N. Jr.
Instituto de Física de Sao Carlos, Universidad de San Pablo, Brasil.

Ortega-Blake, Iván
Instituto de Cs. Físicas (ICF), UNAM, México


National


Bakas,
Laura
Centro de Investigación de Proteínas Vegetales (CIPROVE), Facultad de Ciencias Exactas, UNLP. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. La Plata. prov. de Buenos Aires. Argentina.

Bellini, M. José
Instituto de Investigaciones Bioquímicas de La Plata “Dr. Rodolfo R. Brenner” (INIBIOLP) CONICET-UNLP-CCT La Plata. La Plata. prov. de Buenos Aires.

Fanani, Laura
Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC) -CCT Córdoba. (CONICET-UNC). Provincia de Cordoba. Argentina.

Farina, Mariana
Centro de Estudios Farmacológicos y Botánicos (CEFYBO) -CONICET. Facultad de Medicina-UBA. Ciudad Autónoma de Buenos Aires. Argentina.

Heras, Horacio
Instituto de Investigaciones Bioquímicas de La Plata “Dr. Rodolfo R. Brenner” (INIBIOLP) CONICET-UNLP-CCT La Plata. La Plata. prov. de Buenos Aires.

Herlax, Vanesa
Instituto de Investigaciones Bioquímicas de La Plata “Dr. Rodolfo R. Brenner” (INIBIOLP) CONICET-UNLP-CCT La Plata. La Plata. prov. de Buenos Aires.

Morcelle, Susana
Centro de Investigación de Proteínas Vegetales (CIPROVE), Facultad de Ciencias Exactas, UNLP. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. La Plata. prov. de Buenos Aires. Argentina.

Vairo Cavalli, Sandra
Centro de Investigación de Proteínas Vegetales (CIPROVE), Facultad de Ciencias Exactas, UNLP. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. La Plata. prov. de Buenos Aires. Argentina.

Vela, M. Elena
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA) -CCT La Plata. La Plata. prov. de Buenos Aires. Argentina.

Villa-Abrille, M. Celeste
Centro de Investigaciones Cardiovasculares (CIC) CONICET-UNLP-CCT La Plata. La Plata. prov. de Buenos Aires. Argentina.



Line overview

The main objective of our line of work consists in the study of the lipid-lipid and lipid-protein interactions, determinants of the organization, structure and function of biological membranes. To develop the present line, traditional biophysical and biochemical methodologies are used as well as the more recent methodologies of development, such as atomic force microscope (AFM) and force spectroscopy (FS), surface plasmon resonance (SPR), scale of Langmuir-Brewster angle microscopy (BAM) and polarization modulation-infrared reflection-adsorption spectroscopy (PM-IRRAS), among others. Finally, we develop and give relevant analytic and biophysical methods to research on lipids and membranes. We are eager to share our experience through collaborations with the scientific community.

Research projects

Our current lines of research are focused on problems related to:

  • How lipids interact in model membranes systems and biological membranes to form, or not, laterally segregated domains;
  • How the structure of a sphingomyelin determines its biophysical properties and affects the lipid-lipid interactions;
  • The biological meaning of molecular heterogeneity of sphingolipids;
  • The importance of the asymmetric composition of transmembrane lipids: which its effect in the structure and membrane properties is;
  • How omega-3 fatty acids behave in membranes and how they interact with cholesterol and other lipids;
  • Is there a relationship among the presence of omega-3 fatty acids, the properties of the membrane domains, and the localization and function of Na+/H+ exchanger in cardiac myocytes of hypertensive rats?
  • Is there a relationship among the presence of omega-3 fatty acids, the properties of the membrane domains and the neuroinflammation in hypertensive rats?

Together with Dr. Romina Vázquez and in collaboration with several national and international groups of work, we have been studying the structure and function of lipids domains enriched with cholesterol and sphingolipids. Our studies try to determine, in not only model membrane systems but also biological membranes, the basic principles that promote the formation of such domains and their biophysical properties, as well as the localization-partition-interaction of proteins with them.

A key characteristic of biological membranes is its transversal asymmetry. The cell actively maintains an asymmetric lipid distribution with determined phospholipid classes mainly present in the outer hemilayer and others in the inner membrane hemilayer. Numerous cellular functions including signaling, apoptosis, thrombosis involve an alteration of this asymmetry which is also detected in pathological processes such as inflammation, neurodegenerative processes and cancer. The generation of asymmetric model membrane systems experimentally accessible constitute an important challenge in the field of the membrane biophysics and the main objective of this line of work. Together with the development of asymmetric models, we intend to study the asymmetry impact on the membrane properties, the role of the coupling between hemilayers in the domain formation and its possible role in the signaling, the importance of asymmetry in the interaction of proteins with membrane and in the function of membrane proteins, among others.

* Group directed by Dr. M. Elena Vela (INIFTA-CCT La Plata); Group directed by Dr. Laura Fanani (CIQUIBIC-CCT Córdoba); Group directed by Dr. Mariana Farina (CEFYBO-CONICET);
** Group directed by Dr. Félix M. Goñi (Universidad del País Vasco, España); Group directed by Dr. Osvaldo N. Oliveira Jr. (Instituto de Física de Sao Carlos, Universidad de San Pablo, Brasil); Group directed by Dr. Carlos Muñoz-Garay (Instituto de Cs. Físicas, UNAM, México); Group directed by Dr. Iván Ortega-Blake (Instituto de Cs. Físicas, UNAM, México).

In our laboratory we also work with animal models, studying the effect of diets enriched with omega-3 fatty acids on the formation of membrane domain and the localization-function of membrane proteins within the context of cardiovascular (in collaboration with the laboratory of Dr. M. Celeste Villa-Abrille, CIC, Conicet-CCT La Plata) and neurodegenerative diseases (in collaboration with the laboratory of Dr. M. José Bellini, INIBIOLP, Conicet-CCT La Plata).

Molecules of diverse chemical structure and biological properties interact with the plasmatic membrane to exert their actions or they interact in an unspecific way when reaching the cellular surface. The study the interaction of these molecules (drugs, peptides, proteins, etc.) with model membrane systems enables the evaluation of its potential activity, selectivity, impact on membrane structure and properties, and so on. With different methodologies, we collaborate with different research groups in this type of studies involving surfactants of vegetal origin (in collaboration with Dr. Laura Bakás and Dr. Susana Morcelle del CiProVe, CIC-UNLP), bacterial toxins (in collaboration with Dr. Vanesa Herlax, INIBIOLP, Conicet-CCT La Plata), invertebrate toxins (in collaboration with Dr. Horacio Heras, INIBIOLP, Conicet-CCT La Plata), antimicrobial peptides (in collaboration with Dr. Sandra Vairo Cavalli del CiProVe, CIC-UNLP), antifungal lipoamino acids (in collaboration with Dr. Carlos Muñoz-Garay , Instituto de Cs. Físicas, UNAM, México), among others.

Contact
Prof. Dr. Sabina Maté
Ph.: 54 221 4824894 ext. 103
e-mail: smate@med.unlp.edu.ar      mate.sabina@gmail.com
INIBIOLP (UNLP-CCT La Plata), calle 60 y 120 s/n, La Plata, Buenos Aires, Argentina.

Open posts
Available posts to perform dissertations and periodical fellowship applications for CIC, UNLP, CONICET.

Links
https://www.researchgate.net/lab/Sabina-M-Mate-Lab
https://scholar.google.com/citations?hl=en&user=lCe8mw4AAAAJ

Publications

 

  • “Asymmetric bilayers mimicking membrane rafts prepared by lipid exchange: nanoscale characterization using AFM- Force spectroscopy”.
    Romina F. Vázquez; Erasmo Ovalle-García; Armando Antillón; Iván Ortega-Blake; Laura S. Bakás; Carlos Muñoz-Garay; Sabina M. Maté.
    2021. Biochimica et Biophysica Acta-Biomembranes. Jan 1; 1863(1), 183467
    https://doi.org/10.1016/j.bbamem.2020.183467
  • Exaptation of two ancient immune proteins into a new dimeric pore-forming toxin in Snails”.
    M. L. Giglio; S. Ituarte; V. Milesi; M. S. Dreon; T. R. Brola; J. Caramelo; J. C. H. IpN; S. Maté; J. W. Qiu; L. H. Otero; H. Heras.
    2020. Journal of Structural Biology. Aug 1; 211(2), 107531
    https://doi.org/10.1016/j.jsb.2020.107531
  • “Anandamide regulates oxytocin/oxytocin receptor system in human placenta at term”.
    Paula Accialini; Tomás Etcheverry; Mercedes Negri Malbrán; Gustavo Leguizamón; Sabina Maté; Mariana Farina.
    2020. Placenta. Apr; 93:23-25.
    https://doi.org/10.1016/j.placenta.2020.02.012
  • “Induction of Erythrocyte Microvesicles by Escherichia Coli Alpha Hemolysin”.
    Karen Strack; Natalia Lauri; Sabina María Maté; Andrés Saralegui; Carlos Muñoz-Garay; Pablo J Schwarzbaum; Vanesa Herlax.
    2019. Biochemical Journal. Nov 29; 476(22):3455-3473
    https://doi.org/10.1042/bcj20190546
  • “Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: A PM-IRRAS study”.
    Romina F. Vázquez; Daza Millone María A.; Pavinatto, f; Fanani, María L; Oliveira Jr, Osvaldo n.; Vela, María E; Maté, Sabina.
    2018. Colloids and Surfaces B-Biointerfaces. Jan 1; 173:549-556
    https://doi.org/10.1016/j.colsurfb.2018.10.018
  • “Phase-segregated membrane model assessed by a combined SPR-AFM approach”.
    Daza Millone, María A.; Vázquez, Romina; Maté, Sabina; Vela, María E.
    2018. Colloids and Surfaces B-Biointerfaces.Dec 1; 172:423-429.
    https://doi.org/10.1016/j.colsurfb.2018.08.066 
  • “Volume expansion of erythrocytes is not the only mechanism responsable for the protection by Arginine-based surfactants against hypotonic hemolysis”.
    María E. Fait; Melisa Hermet; Romina Vázquez; María A. Daza Millone; Sabina Maté; María E. Vela; Susana Morcelle; Laura Bakas.
    2018. Colloids and Surfaces B-Biointerfaces. Nov 1; 171:134-141
    https://doi.org/10.1016/j.colsurfb.2018.07.005
  • Dynamic regulation of extracellular ATP in Escherichia coli”.
    Cora L Alvarez; Gerardo Corradi; Natalia Lauri; Irene Marginedas Freixa; María F Leal Denis, Nicolás Enrique; Sabina M Maté; Verónica Milesi; Mariano A Ostuni; Vanesa Herlax; Pablo Schwarzbaum.
    2017. Biochemical Journal. Apr 4; 474(8):1395-1416
    https://doi.org/10.1042/BCJ20160879
  • “Interaction of acylated and unacylated forms of E. coli alpha-hemolysin with lipid monolayers: a PM-IRRAS study”.
    Romina F. Vázquez; María A. Daza Millone; Felippe J. Pavinatto; Vanesa S. Herlax; Laura S. Bakás; Osvaldo N. Oliveira Jr; María E. Vela; Sabina M. Maté.
    2017. Colloids and Surfaces B-Biointerfaces. Jun 24; 158:76-83
    https://doi.org/10.1016/j.colsurfb.2017.06.020
  • “Relationship between intracellular calcium and morphologic changes in rabbit erythrocytes: Effects of the acylated and unacylated forms of E. coli alpha-hemolysin”.
    Vázquez RF; Maté SM; Bakás LS; Muñoz-Garay C; Herlax VS.
    2016. Biochimica et Biophysica Acta-Biomembranes. Aug; 1858(8):1944-53
    https://doi.org/10.1016/j.bbamem.2016.05.013
  • “The unfavourable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas”.
    Levi Lorena; Castro-Parodi Mauricio; Martínez Nora; Piehl Lidia; Rrubín de Celis Emilio; Herlax Vanesa; Maté Sabina; Farina Mariana; Damiano Alicia.
    2016. Biochimica et Biophysica Acta-Biomembranes. Sep; 1858(9):2171-2180
    https://doi.org/10.1016/j.bbamem.2016.06.014 
  • “Induction of eryptosis by low concentrations of E. coli alpha-hemolysin”.
    Fernanda Carrizo Velasquez; Sabina Maté; Laura Bakás; Vanesa Herlax.
    2015. Biochimica et Biophysica Acta-Biomembranes. Nov; 1848(11 Pt A):2779-88
    https://doi.org/10.1016/j.bbamem.2015.08.012 
  • “Boundary region between coexisting lipid phases as initial binding sites for Escherichia coli alpha hemolysin: a real-time study”.
    Sabina María Maté; Romina F Vázquez; Vanesa S Herlax; María A Daza Millone; María L Fanani; Bruno Maggio; María E Vela; Laura S Bakas.
    2014. Biochimica et Biophysica Acta-Biomembranes. Jul; 1838 (7): 1832–1841
    https://doi.org/10.1016/j.bbamem.2014.02.022
  • “N-nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes”.
    Sabina Maté; Jon V. Busto; Aritz B. García-Arribas; Jesús Sot; Romina Vazquez; Vanesa Herlax; Claude Wolf; Laura Bakás; Félix M. Goñi.
    2014. Biophysical Journal. Jun 17; 106(12):2606-16.
    https://doi.org/10.1016/j.bpj.2014.04.054
  • Novel evidence for the specific interaction between cholesterol and alpha hemolysin of E. coli
    Romina Vazquez; Sabina Maté; Laura Bakás; Marisa Fernandez; Emilio Malchiodi; Vanesa Herlax.
    2014. Biochemical Journal. Mar 15; 458(3): 481-489
    https://doi.org/10.1042/BJ20131432
  • Mecanismo de acción de la toxina alfa hemolisina de Escherichia Coli. 
    Bakás, L; Maté, S; Vázquez, R; Herlax, V. 
    2013
    . Acta bioquímica clínica latinoamericana. La Plata: Federación BioquímicaProvincia Buenos Aires. Vol. 47, n° 2, p. 353-361. ISSN 0325-2957


  • E.coli Alpha Hemolysin and properties 
    Bakás, L; Maté, S; Vazquez, R; Herlax, V.
    2012. Biochemistry, BooK I, Ed. Prof. Deniz Ekinci, Croacia. 

    http://www.intechopen.com/articles/show/title/e-coli-alpha-hemolysin-and-properties

  • Relevance of fatty acid covalently bound to Escherichia coli alpha-Hemolysin and membrane microdomains in the oligomerization process. 
    Herlax, V; Maté, S; Rimoldi, O; Bakas, L. 
    2009. Journal of Biological Chemistry. Amer Soc Biochemistry Molecular Biology, Inc, vol. 284, p. 25199-25210

Book - Book chapters

  • “Role of sphingomyelin in membrane-domain formation and the influence on protein interaction: focusing on the nanometer scale”.
    Sabina Maté; Vanesa Herlax; Romina Vázquez; Laura Bakás
    2016. Cap 5: 89-112 en Membrane organization and lipid rafts in the cell and artificial membranes, Ed. Dr. Catalá, A. Nova SciencePublishers.  ISBN: 978-1-63484-589-2
  • Alfa- Hemolisina de Escherichia coli: Prototipo de las toxinas RTX (Repeat in Toxin). Un estudio de las etapas de su mecanismo de acción.
    Vázquez, R; Maté, S; Herlax, V; Bakás, L; Álvarez Valcárcel, C; Pazos Santos, F.
    2016. Puebla: Benemérita Universidad Autónoma De Puebla, p. 191-213. ISBN 978-607-525-046-5


  • E. coli Alpha Hemolysin and Properties. 
    Bakás, L; Maté, S; Vázquez, R; Herlax, V.
    2012. InTech, p. 107-140. ISBN 978-953-51-0076-8




INIBIOLP - Instituto de Investigaciones Bioquímicas de La Plata "Profesor Doctor Rodolfo R. Brenner"
Consejo Nacional de Investigaciones Científicas y Técnicas - Facultad de Ciencias Médicas - U.N.L.P.
Avenida 60 y 120 - C.P. 1900 La Plata - Prov. de Buenos Aires - Argentina. Tel. 54-221-4824894 - Fax. 54-221-4258988 - E-mail :