The High-standard Technological services (STANs) comprise activities such as assays, technical assistance and training, for which the equipment, infrastructure and human resources are granted by INIBIOLP. STANs are offered to several areas of society.
STAN Specifications: Analysis of fatty acids by gas chromatography.
Methodology: Analysis by CGC: An Omega Wax 250 column of 30 m will be used.
The operative conditions will be as follows: injector in split mode at 260 260°C, programmed oven temperature, and flame ionization detector will be maintained at 280°C.
Helium will be the gas carrier at a flux of 1-2 ml/min.
Equipment: Gaseous Chromatograph Hewlett Packard 6890.
STAN Specifications: Analysis by gaseous chromatography – mass spectrometry for the detection / identification of compounds or organic contaminants.
Methodology: 1) Determination of optimal operational conditions of sample nature. Sample taking (liquid or gaseous) and injection. Deliver of chromatogram and spectra of interest in printed or electronic version.
2) Identity assignment by comparison of spectra obtained with spectra commercial libraries (NIST 05/EPA/NIH, pesticides, essential oils and others) and with other spectra obtained from standards (provided by the applicant).
Equipment: Capillar gas chromatograph with spectrometry detector of mass Agilent Technologies CGC-HP6890 MS5975VL.
STAN Specifications: Fractioning of biological samples by differential ultracentrifugation.
Methodology: The fractioning of biological samples will be performed previously to adequate rotor selection and rotation velocity, solvent density, etc.
Installation startup, sample loading, and ultracentrifugation.
Equipment: Beckman Coulter LE-80K Preparative Ultracentrifuge.
STAN Specifications: Evaluation of insecticide capacity. Analysis of insecticide efficacy of biological, physical or chemical insecticide agents by means of mortality determination, effect on life cycle of insects, etc, and/or evaluation of lethal doses.
Methodology: Bioassays will be performed by exposing insects to compounds to be evaluated. The application mode depends on the physicochemical properties of the compound and/or as it was solicited. Batches of at least 10 insects will be employed, with at least 4 replicas for each concentration or condition to be assayed. Statistical analysis of the results.
STAN Specifications: Protein purification by molecular exclusion and ion exchange.
Methodology: 1)Protein purification by ion Exchange:
It is used an ion exchanger column (Mono-Q 10/100 GL) that separates proteins based upon its superficial net charge at a determined pH value. The different gradients of increasing ionic strength affect the interaction between the filling and the biomolecules, thus achieving their separation. Protein detection is performed by absorbance at 280 nm. Brenner
2)Protein purification by molecular exclusion:
It is used molecular exclusion columns capable of separating globular proteins in a range of molecular masses between 3,000 and 70,000 Da (Superdex 75 10/300GL) and between 100,000 and 600,000 Da (Superdex 200 10/300 GL). The mobile phase is an aqueous buffer which is isocratically used. The presence of proteins in the column eluent is detected by absorbance at 280 nm.
Equipment: Acquity Arc System for HPLC and UHPLC
STAN Specifications: Determination of drug or compound toxicity in cell culture
Methodology: The basic service includes the cell incubation with the drug o compound of interest, its staining with crystal violet or the determination of the mitochondrial activity by standard colorimetric methods (3-(4,5-dimetylthiazol)-2,5-diphenyltetrazolium (MTT) bromide) in order to evaluate the effect of the drug/compound on the cell proliferation/viability. Both the cell line and the experimental design to be used will be coordinated with the user based on the availability of the institute and on the requirements of the study to be carried out.
The statistical analysis of the results, the LD50 determination and other more specific analysis are not included in the basic service, but they can be coordinate with the user.
Equipment: BSC Esco class II Laminar Flow BSC Model ACZ-451, Panasonic CO2 Laboratory Incubator Model MCO-18AC-PE, Beckman Coulter DTX 880 Multimode Detector Plate Reader.
STAN Specifications: Real-time quantitative PCR analysis.
Methodology: The service includes the relative quantification of genes of interest, normalized with reference genes in problem samples compared to control samples. It can include, on the request of the interested party, the previous nucleic acid extraction (RNA, DNA), the cDNA synthesis, the construction of calibration curves, and the data statistical analysis.
Equipment: Real-Time PCR Agilent Technologies Aria Mx
STAN Specifications: System for the quantification of equivalent genomes of virus SARS-COV-2 in naso-pharyngeal swab samples and other biological samples (RUO).
Methodology: It consists of a one-step qRT-PCR (R.U.O.) assay that allows the accurate detection and quantification of SARS-CoV-2 viral load through the analysis of two viral genes (ORF1ab and E gene) and a reference gene (RNAseP) on viral RNA samples obtained from several biological samples. The samples may consist of naso-pharyngeal swab tests, cerebrospinal fluid (CSF), saliva, fecal matter, blood or any other human or animal biological sample, or of inert material such as raw sewage, where virus SARS-CoV-2 can be detected. These biological samples will be provided by the contracting party according to their study design. All the material will be handled according to the Biosecurity Compliance Requirements. The contracting party will receive a report with the results to be used as epidemiologic diagnosis and/or analysis in accordance with the purpose of the sample derivation.
STAN Specifications: The basic service consists of collecting data from biological samples using the flow cytometer. The samples can be fresh or fixed in tubes, eppendorf tubes or multiwall plaques. Functional studies can also be performed; namely, to monitor the cell response over time against a stimulus. Besides, the processing and marking of biological samples can be sought in order to acquire and/or carry out the analysis of the obtained data.
Equipment: BD Accuri C6 Plus Flow Cytometer: it has two lasers, blue (488 nm) and red (630 nm). 6 parameters can be measured: FSC, SSC and 4 fluorescence channels (510nm-800 nm).
Chief technician: Julia Tau, support personnel, CONICET.
STAN Specifications: Optimization of culture conditions for the growth and differentiation of several types of fungal propagules (conidia, blastopores, mycelial pellets, microsclerotia) to be used as biosupplies in the handle of plague insects, from strains of fungi provided by the contracting party. Planning and monitoring of biochemical and molecular markers useful as parameters of virulence. Evaluation of the advantages and disadvantages of the different types of propagules.
Methodology: Practical consulting (face-to-face workshops) at the requested laboratory and theoretical consulting with talks (face-to-face or virtual) will be provided. Consulting is tailored to both personnel of public institutions such as universities and laboratories from state institutions and private companies interested in the development and production of bioinsecticides products.
STAN Specifications: Quantification and analysis of samples of nucleic acids through nano-spectrophotometry followed by electrophoresis
Methodology: The service consists of two stages which can be requested together or separately:
1) Precise quantification of DNA, RNA, from 1-2 µL of the sample with a nano-spectrophotometer and analysis of their quality parameters through the relation between the absorvances 260nm/280nm and 260nm/230nm;
2) Visualization, quality control and product detection of DNA, RNA through agarose gels electrophoresis. Gel visualization is performed by a UV transilluminator.
Equipment: Nanodrop Thermo SCIENTIFIC, 2000c Spectophotometer; electrophoresis cube Amersham Biosciences, HE33 mini horizontal submarine unit.